中华医学杂志（英文版）2001，Vol.114Issue(8)：560-863,304.
小鼠抗E2抗体在体外可以捕获丙型肝炎病毒
Murine antibody against E2 can capture hepatitis C virus in vitro
马文彬 1冯百芳 1陶其敏1
作者信息
- 1. 北京大学人民医院肝病研究所北京 100044
- 折叠
摘要
Abstract
Objective To find neutralizing antibody candidates against hepatitis C virus (HCV) infection.
Methods We constructed two eukaryotic expression vectors which contained the E1 and E2 gene of HCV, and detected their expression in mammalian cells with transient expression. BALB/c mice were given subculaneous injections of constructed vectors combined with the IL-2 gene intraepidermally and evaluated for induced humoral immune responses by enzyme-linked immunosorbent assay (ELISA). We used an antibody-virus interaction assay to analyze the interaction of the antisera and HCV viral particles in vitro.
Results Anti E1 and anti-E2 antisera were obtained from immunized mice. The serum of mice immunized with the E2 gene immunoprecipitated the HCV isolate in source serum and reacted with the isolates unrelated to the original one.
Conclusions Anti-E2 antibody in induced mice can cross-reactively capture HCV particles, highlighting the possibility of generating broadly reactive anti-E2 antibodies.关键词
丙型肝炎病毒/中和抗体/包膜蛋白Key words
hepatitis C virus/neutralizing antibody/envelop protein分类
医药卫生引用本文复制引用
马文彬,冯百芳,陶其敏..小鼠抗E2抗体在体外可以捕获丙型肝炎病毒[J].中华医学杂志（英文版）,2001,114(8):560-863,304.
