植物学通报2003，Vol.20Issue(5)：565-571,7.
青阳参组织培养及愈伤组织的成分分析
Studies on the Tissue Culture of Cynanchum otophyllum and Calli Chemical Constituents
摘要
Abstract
The calli of Cynanchum otophyllum were induced from the tender stems and young buds on MS medium with the supplement of 2.0 mg/L 2,4-D and 0.1 mg/L KIN. The result showed that 6,7-V medium was the optimal medium for callus growth. The tests using plant growth regulators with various concentrations showed that 6,7-V medium plus 2.0 mg/L 2,4-D and 0.3 mg/L KIN was the best combination for callus growth, whereas the production of secondary metabolites was higher on 6,7-V medium plus 1.0 mg/L 2,4-D and 0.1 mg/L KIN. The best period of culture was 27 d for callus growth, and 33 d for the maximal production of secondary metabolites. The isolation of nature products from the callus provided seven compounds including (1) fatty acid methyl ester named methyl 9,10,11-trihydroxy-12-octadecenoate and (2) daucosterol, (3) β-sitosterol, (4) betulinic acid, (5) oleanolic acid, (6) hexadecanoic acid, (7) 9-octadecenoic acid. This is the first report of the isolation of poly-hydroxyl octadecenoic acid from a callus, and its proven biological function on the growth of plant cells was discussed.关键词
9,10,11-三羟基-十八碳-12(Z)-烯酸甲酯,青阳参,愈伤组织,萝(艹摩)科Key words
Methyl 9,10,11-trihydroxy-12-octadecenoate, Cynanchum otophyllum, Callus, Asclepiadaceae分类
农业科技引用本文复制引用
赵沛基,甘烦远,珠娜,沈月毛..青阳参组织培养及愈伤组织的成分分析[J].植物学通报,2003,20(5):565-571,7.基金项目
国家自然科学基金(30070007)和云南省科学基金(99B0017G)资助项目 （30070007）
