动物营养学报2009，Vol.21Issue(4)：567-572,6.DOI:10.3969/j.issn.1006-267x.2009.04.023
谷氨酰胺对断奶仔猪抗菌肽PR-39 mRNA的表达调控
Effects of Glutamine on Antibacterial Peptide PR-39 mRNA Expression in Weaned Piglets
摘要
Abstract
To investigate the effects of glutamine(Gln) on expression of antibacterial peptide PR-39 mRNA in weaned piglets.Sixty four 28-day-old weaned piglets (Duroc×Landrace×Yorkshire) with an initial body weight of (7.50±0.67) kg were randomly allocated to two groups (control group and 1.0% Gin-added group) with four replicates in each group of eight per replicate (half male and half female).On day 28, the levels of IL-1β, IL-6 and TNF-α were analysed.On day 30, the effects of Gin on expression of antibacterial peptide PR-39 mRNA were determined by semi-quantitative RT-PCR.The re-sults indicated that, the level of IL-1β in serum increased significantly (P<0.05) with Gin addition, however, the levels of IL-6, TNF-α in serum and TNF-α, IL-2 in mucosal of jejunum were not significant affected with Gin addition compared with control group (P>0.05); the relative expressions of PR-39 mRNA in femur marrow and mucosal of jejunum signifi-cantly increased by 50.5% (P<0.05) and 24.0% (P<0.05), respectively.It was concluded, addition of Gin to diets for weaned piglets could significantly increase relative expression of PR-39 mRNA in both femur marrow and mucosal of jeju-num.关键词
谷氨酰胺/断奶仔猪/抗菌肽/PR-39mRNA/基因表达Key words
Glutamine/Weaned piglets/Antibacterial peptide/PR-39 mRNA/Gene expression分类
农业科技引用本文复制引用
赵玉蓉,王红权,贺建华,范志勇..谷氨酰胺对断奶仔猪抗菌肽PR-39 mRNA的表达调控[J].动物营养学报,2009,21(4):567-572,6.基金项目
湖南省重大科技专项(2007FJ1003) （2007FJ1003）
湖南省自然科学基金项目(06JJ50070) （06JJ50070）
湖南农业大学人才引进基金项目(YJ0508) （YJ0508）
