首页|期刊导航|中国农业科学（英文版）|Effects of Auxins and Media on Callus Induction of Chinese Spring Wheat( Triticum aestivum L.)
中国农业科学（英文版）2005，Vol.4Issue(8)：567-573,7.
Effects of Auxins and Media on Callus Induction of Chinese Spring Wheat( Triticum aestivum L.)
Effects of Auxins and Media on Callus Induction of Chinese Spring Wheat( Triticum aestivum L.)
摘要
Abstract
The effects of auxins and media on callus induction from the mature and immature embryos of Chinese spring wheat(Triticum aestivum L.) varieties were investigated. It was found that genotype, medium, auxin source and concentration had the significant effects on the induction of embryogenic callus, explants germination and the increment of callus fresh weight. For immature embryos cultured on MS medium, 2 mg L-1of 2, 4-D was optimal, and the highest frequency of embryogenic callus (33.50%) was observed. For the mature embryos on N6 medium, 4 mg L-1 of 2, 4-D was optimal. The frequency of embryogenic callus and increment of callus fresh weight on 2, 4, 5-T media were higher than those on 2, 4-D media, and in the presence of 2, 4, 5-T the precocious germination of explants for all genotypes were significantly suppressed. These results indicated that 2, 4, 5-T was superior to 2, 4-D and NAA in the culture of immature embryos. This is the first report about the effect of 2, 4, 5-T and NAA on wheat tissue culture, particularly in comparison with 2, 4-D in detail.关键词
Auxin/Embryogenic callus/Germination frequency/Fresh weight/Wheat (Triticum aestivum L.)Key words
Auxin/Embryogenic callus/Germination frequency/Fresh weight/Wheat (Triticum aestivum L.)分类
农业科技引用本文复制引用
WU Li-min,WEI Yu-ming,ZHENG You-liang..Effects of Auxins and Media on Callus Induction of Chinese Spring Wheat( Triticum aestivum L.)[J].中国农业科学（英文版）,2005,4(8):567-573,7.基金项目
This work was supported by the National High Tech R&D Program of China (863 Program, 2003AA207100)and the Foundation for the Author of National Excellent Doctoral Dissertation of P R China (200357). （863 Program, 2003AA207100）
