中国临床康复2004，Vol.8Issue(26)：5681-5683,3.
淫羊藿总黄酮对切除卵巢大鼠发生骨质疏松症的抑制作用
Inhibitory effect of total flavonoid extract of Epimedium sagittatum on rat osteoporosis induced by ovariectomy
摘要
Abstract
BACKGROUND: Epimedium herb has been one of the most frequently used herbs in treating osteoporosis in traditional Chinese medicine. However. its anti-osteoporotic constituents have rarely been studied.OBJECTIVE: To investigate whether the total fiavonoid extract(TFE) of Epimedium sagittatum has an anti-osteoporosis activity.DF SIGN: A completely randomized, auto-controlled and mutual controlled study was used.SETTING and PARTICIPANTS: Forty-eight adult female SD rats provided by the Department of Animal Experiment of Lanzhou General Hospital Area Command of Chinese PLA. were used and the experiment was completed in the Department of Animal Experiment of Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA.INTERVENTIONS: Forty-eight rats were divided into 6 groups and underwent bilateral ovariectomy (OVX)or sham operation. TFE were orally administered at 50, 100 and 200 mg/kg per day separately. Nilestriol(E2) was given at 1.5 mg/kg per day and used as positive control. All rats were sacrificed after three months.MAIN OUTCOME MEASURES: The biochemical and bone histomorphometric pararmeters, bone mineral density(BMD) and breaking strength of femurs were measured.RESULTS: OVX caused bone micro architectural deterioration and significant decreases in both of the bone mass and mechanical strength of femur. The serum alkaline pbosphatase(ALP) [(219.41 ±34.52) U/L] in OVX was obviously higher than that in normal control group; TFE200 and E2, (164.32 + 37.96) and(150. 30 ± 44. 83) U/L respectively, were obviously lower than those of the OVX group; the level of serum isteocalcin(OC)was similar to OVX group. Urine Ca/Cr, P/Crand DPD/Cr was obviously higher than that of the normal control group; All the markers of E2 group were lower than those of OVX group( t =4. 17 -4. 50, P ＜ 0. 05) . The BMD of femur in OVX was obviously lower than in normal control group. Each marker of TFE200 and E2 were obviously higher than those of OVX group. Change in Lumbar vertebrae of L2- 4 was similar to that in femur. TFE inhibited or attenuated the effect of OVX, especially at 200 mg/kg per day, which exerted almost the same preventive effect as nyestriol without exhibition of uterotrophic activity.CONCLUSION: TFE has an anti-osteoporotic activity.关键词
骨质疏松/淫羊藿/黄酮/卵巢切除术分类
医药卫生引用本文复制引用
陈克明,葛宝丰,马慧萍,白孟海,王勇,刘剑梅,高梅..淫羊藿总黄酮对切除卵巢大鼠发生骨质疏松症的抑制作用[J].中国临床康复,2004,8(26):5681-5683,3.基金项目
全军"十五"期间重大课题(010Z012) （010Z012）
