中国药科大学学报2006，Vol.37Issue(6)：569-572,4.
用Cy5-ddNTP结合修饰引物延伸测定人类3种SNP
Genotyping three human SNPs by Cy5-ddNTP primer extension
宋沁馨 1周国华2
作者信息
- 1. 中国药科大学药物分析教研室,南京210009
- 2. 南京军区军事医学研究所,南京,210002
- 折叠
摘要
Abstract
To establish a simple and rapid method to genotype the human single nucleotide polymorphisms(SNPs). Methods:In the presence of the appropriate Cy5-ddNTPs and dNTPs,the primer was extended by one or more bases depending on the sequence at the polymorphic site. The microchip electrophoresis was used to detect the product of extension.The switching characteristics of the primer extension reaction was increased by introducing an artificially mismatched base into the third position from the 3'-terminus of the primers. Results:Three human SNPs were detected and the alleles were distinguished on the basis of size of the extended product. All samples were accurately assayed within 100 s in microchip electrophoresis. Conclusion:This technique offers a high-speed,easy and cheap method for genotyping SNPs.关键词
SNP/Cy5-ddNTP/芯片电泳/修饰引物延伸Key words
SNP/Cy5-ddNTP/microchip electrophoresis/modified primer extensio分类
生物科学引用本文复制引用
宋沁馨,周国华..用Cy5-ddNTP结合修饰引物延伸测定人类3种SNP[J].中国药科大学学报,2006,37(6):569-572,4.
