中华医学杂志（英文版）2001，Vol.114Issue(6)：571-576,6.
芦荟大黄素对动脉损伤后平滑肌细胞PCNA基因表达的影响
Effect of aloe-emodin on expression of proliferating cell nuclear antigen of vascular smooth muscle cells in culture after arterial injury
徐成斌 1王申五 2尹春琳3
作者信息
- 1. 北京医科大学人民医院心内科北京 100044
- 2. 北京医科大学人民医院中心实验室北京 100044
- 3. 北京市宣武医院心内科北京 100053
- 折叠
摘要
Abstract
Objective To observe the effect of aloe-emodin on the expression of proliferating cell nuclear antigen (PCNA) in smooth muscle cells (SMCs) after arterial injury and study the molecular mechanism of inhibition of aloe-emodin on SMC proliferation.
Methods Deendothelialization was performed at the abdominal aorta in Japanese white rabbits using a 3F Fogarty arterial embolectomy catheter. 48 hours later, the medium of abdominal aorta was isolated and primary SMCs culture was performed. Cells were synchronized to G0 by serum starvation, then aloe-emodin at a concentration of 20?μg/ml was added to the culture medium containing 10%［v/v］ fetal calf serum. Vehicle was also added to the medium as a control. After 18 hours, the expression of PCNA at the level of mRNA and protein were examined using techniques of RT/PCR, Western blotting and inmmunocytochemistry respectively.
Results Compared with the control group, the expression of PCNA mRNA and protein was prominently decreased after addition of aloe-emodin.
Conclusion The inhibition of aloe-emodin on SMCs proliferation may be caused by inhibiting the expression of the PCNA gene.关键词
增殖细胞核抗原/芦荟大黄素/平滑肌细胞Key words
proliferating cell nuclear antigen/aloe-emodin/smooth muscle cell分类
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徐成斌,王申五,尹春琳..芦荟大黄素对动脉损伤后平滑肌细胞PCNA基因表达的影响[J].中华医学杂志（英文版）,2001,114(6):571-576,6.
