首页|期刊导航|中国农业科学（英文版）|Characterization of Citrus tristeza virus Isolates by Indicators and Molecular Biology Methods
中国农业科学（英文版）2007，Vol.6Issue(5)：573-579,7.
Characterization of Citrus tristeza virus Isolates by Indicators and Molecular Biology Methods
Characterization of Citrus tristeza virus Isolates by Indicators and Molecular Biology Methods
摘要
Abstract
Citrus tristeza virus (CTV) exists in citrus as a large number of distinct strains differing in biological characters. The control strategies such as mild strains cross protection (MSCP) require a clear understanding of the characterization of CTV. For better understanding of the structure of CTV population and the relationship between molecular and biological characterization, 72 CTV samples collected from five provinces in China were studied, using biological indexing, p25/Hinf Ⅰ restriction fragment length polymorphism (RFLP), multiple molecular markers, and bidirectional RT-PCR assay. The mixture of severe stem pitting isolates was found to be dominant in the field. CTV isolates with p25/Hinf Ⅰ RFLP group3 and p23/BD-PCR group Ⅰ, Ⅲ were the main cause of epidemics, and most CTV isolates were found to be the mixture of T30 and VT genotypes. More accurate identification of strain mixtures in the field and better understanding of the biological traits of the isolates may be achieved by applying the three molecular detection methods simultaneously.关键词
Citrus tristeza virus (CTV)/biological indexing/molecular detectionKey words
Citrus tristeza virus (CTV)/biological indexing/molecular detection分类
农业科技引用本文复制引用
ZHOU Yan,ZHOU Chang-yong,SONG Zhen,LIU Ke-hong,YANG Fang-yun..Characterization of Citrus tristeza virus Isolates by Indicators and Molecular Biology Methods[J].中国农业科学（英文版）,2007,6(5):573-579,7.基金项目
This study was supported by National Natural Science Foundation of China (30471205). （30471205）
