中国农业科学（英文版）2005，Vol.4Issue(8)：574-578,5.
Transformation of TrxS Gene into Barley by Particle Bombardment
Transformation of TrxS Gene into Barley by Particle Bombardment
摘要
Abstract
The production of malting barley in China can't meet the demand of beer industries because of poor quality and it becomes a bottleneck problem in beer manufacture industry. In this paper, TrxS gene cloned from Phalaris coerulescens was transferred into barley cultivar Yupi 1 (YP1)via biolistic bombardment. 1206 immature embryos were bombarded and seven transgenic plants carrying TrxS gene were confirmed by PCR and PCR-Southern blotting analysis. TrxS gene was expressed in transgenic plants by RT-PCR analysis. The activity of Trxh and αt-amylase of transgenic line were higher than that of non-transgenic line, which is helpful to improve malting quality of barley.关键词
Barley/TrxS gene/Particle bombardment/TransformationKey words
Barley/TrxS gene/Particle bombardment/Transformation分类
农业科技引用本文复制引用
WEI Li,YIN Jun,KONG Wei-wei,REN Jiang-ping,LI Lei,LIU Lei..Transformation of TrxS Gene into Barley by Particle Bombardment[J].中国农业科学（英文版）,2005,4(8):574-578,5.基金项目
The project was supported by the National Special Program for Transgenic Plants Research (JY2003-B-20-3). （JY2003-B-20-3）
