作物学报2000，Vol.26Issue(5)：575-578,4.
甘蓝型油莱Pol CMS育性恢复基因的RAPD标记
Identification of RAPD Markers Linked to the Fertility Restoring Gene for the Polima CMS in Rapeseed (Brassica napus L.)
摘要
Abstract
Bulked segregant analysis was used to identify random amplified polymorphic DNA(RAPD) markers linked to the fertility restoring gene for the Polima cytoplasmic male sterility (Pol cms) in rapeseed (B. napus L.). Totally 860 random 10-mer primers were screened on the DNA samples of fertile and sterile bulks. Two primers, AH19 and AIl6, produced repeatable polymorphic bands between the paired bulks. DNA samples from individual plants of a back cross population (1141A/Hui5200)//1141B)were then used as DNA samples template for PCR amplification with these two primers.RAPD data was analyzed with program of Joinmap.The results indicated that the RAPD markers, AH19690 and AI16830 were linked to the Pol cms Rf gene at one side, with a map distance of 5.9 cM and 13.6 cM, respectively.The distance between the two markers was 7.8 cM.关键词
甘蓝型油菜/波里马细胞质雄性不育/恢复基因/RAPD标记/集团分离分析Key words
Brassica napus/Polima cytoplasmic male sterility/Restoring gene/RAPD markers/Bulked segregant analysis分类
农业科技引用本文复制引用
王俊霞,杨光圣,傅廷栋,孟金陵..甘蓝型油莱Pol CMS育性恢复基因的RAPD标记[J].作物学报,2000,26(5):575-578,4.基金项目
国家高技术研究发展计划(101-02-03-01) （101-02-03-01）
