中国组织工程研究与临床康复2009，Vol.13Issue(3)：575-578,4.
聚乳酸-羟基乙酸包载眼镜蛇毒细胞毒素缓释微球的表征及体外释放行为
Superficial property and drug release in vitro of poly lacticoglycolic acid-loaded cytotoxin nanoparticles
陈志奎 1林礼务 1张志强 2薛恩生 1王艳 1俞丽云1
作者信息
- 1. 福建医科大学附属协和医院超声科,福建省福州市,350001
- 2. 福建医科大学医药生物工程中心,福建省福州市,350001
- 折叠
摘要
Abstract
BACKGROUND: Cytotoxin (CTX) is characterized by strong cytotoxic activity and absent specificity, of which systemic administration can cause severe toxicity and side effects; however, interstitial chemotherapy may improve therapeutic effect of local tumor and relieve general toxicity using sustained release vector. OBJECTIVE: To prepare cytotoxin/poly lacticoglycolic acid (CTX-PLGA) nanoparticles, and to study the general property and drug release in vitro. DESIGN, TIME AND SETTING: Observational study was performed at Medical Bicangineedng Center of Fujian Medical University between December 2007 and May 2008. MATERIALS: Poly lacticoglycolic acid (PLGA) and polyvinyl alcohol were purchased from Chengdu, and CTX was purchased from Guangdong. METHODS: CTX was purified by molecular sieve, ion exchange separation, and reversed-phase high performance liquid chromatography; cytotoxic activity was measured by MTT method; nanoparticles were prepared by multiple emulsion-solvent evaporation technique. MAIN OUTCOME MEASURES: Superficial morphological characteristics were observed using scanning electron microscope, while diameter was measured using laser particle sizing analyzer in order to calculate encapsulation efficiency, drug loading, and release cycle in vitro. RESULTS: Purified CTX had apparent cytotoxic activity, and the IC<,50> values of HepG2 cells were respectively 1.43 and 1.12 mg/L at 12 and 24 hours. By using multiple emulsion-solvent evaporation technique, the nanoparticles were smooth and intact, diameter 2-8 μ m. The encapsulation efficiency was (74.10±9.92)%, and the drug loading was (0.72±0.09)%. In addition, drug accumulated release was 63.3% at day 21. The released CTX still had a great biologic activity. CONCLUSION: Multiple emulsion-solvent evaporation technique is surely prepared the CTX-PLGA nanoparticles, characterizing by high encapsulation efficiency, great drug release, and entire biologic activity.关键词
细胞毒素/聚乳酸-羟基乙酸微球/缓释分类
基础医学引用本文复制引用
陈志奎,林礼务,张志强,薛恩生,王艳,俞丽云..聚乳酸-羟基乙酸包载眼镜蛇毒细胞毒素缓释微球的表征及体外释放行为[J].中国组织工程研究与临床康复,2009,13(3):575-578,4.
