昆虫学报2008，Vol.51Issue(6)：575-579,5.
饲料中维生素和无机盐对rBmNPV-Bm表达系统植酸酶基因表达活性的影响
Effects of vitamins and inorganic salt in artificial diet on the expression of phytase gene in rBmNPV-Bm expression system
摘要
Abstract
[Aim]Enhancing the yield of silkworm bioreactor and disclosing the relation between the host nutritive condition and the expression of foreign gene in rBmNPV-Bm system.[Methods]The effects of vitamins and inorganic salt in artificial diet for the host insect of baculovirus.silkworm Bombyx mori,on the activity of foreign gene products was studied using BmNPV as an expression vector and phytase as a reporter gone.[Results]The activity of phytase in silkworm haemolymph varied remarkably with the amount of added vitamin and inorganic salt.The phytase activity in silkworm haemolymph reached the maximum when the amount of vitamin C was 1%of the dry weight of artificial diet,vitamin B mixture 0.25%,inorganic salts mixture 1% and the amount of vitamin B6 is 2 mg per 100 g dry matter weight,respectively in the designed test scope.[Conclusions]The expression of phytase gene in BmNPV-Bm expression system could be enhanced efficiently by altering the levels of micronutrients available in the artificial diet for silkworm larvae.关键词
BmNPV-Bm表达系统/维生素/无机盐/植酸酶基因/表达/酶活性Key words
BmNPV-Bm expression system/vitamin/inorganic salt/phytase gene/expression/enzyme activity分类
生物科学引用本文复制引用
尚金燕,王冰,崔为正,吴小锋,张志芳..饲料中维生素和无机盐对rBmNPV-Bm表达系统植酸酶基因表达活性的影响[J].昆虫学报,2008,51(6):575-579,5.基金项目
Project of Shandong Provincial Education Department Foundation(J01I01) （J01I01）
