北京林业大学学报2001，Vol.23Issue(2)：57-60,4.
绿色木霉纤维素酶AS3.3032固态发酵的研究
Production of cellulase by Trichoderma viride AS3.3032 in sloid state fermentation
摘要
Abstract
In the research, using wheat bran and steam exploded bagasse asmain substrate, cellulase is produced by solid state fermentation with Trichoderma viride AS3.3032. The effect on cellulase production is studied of nitrogen source, carbon source, surface active agent, inoculation, temperature, wate content and initial pH value of solid substrate. The results are:①With NH4SO4 as nitrogen source, FPA,CMC and β-gase all are high, 122.5 FPA U/g dried substrate, 1470.0 CMC U/g dried substrate and 119.3 β-Gase U/g dried substrate respectively;②With wheat bran and bigasse(their ratio 3 to 2) as carbon source, FPA, CMC and β-Gase get highest, 138.2 FPA U/g dried substrate, 134.6 β-Gase U/g dried substrate and 1603.1 CMC U/g dried substrate respectively;③Adding 0.1% Tween-80 to culture medium, FPA, β-Gase and CMC can increase by 230%,280% and 230% respectively, but with addition of 0.5%～0.7% washing powder, the three above indices increase by 310%,370%,300% resepctively;④The activity of produced cellulase goes up to the highest when the water amount of medium, the temperature and the initial pH are 250%,28℃ and 3.5.关键词
纤维素酶/固体发酵/酶活力分类
生物科学引用本文复制引用
张德强,黄镇亚,张志毅..绿色木霉纤维素酶AS3.3032固态发酵的研究[J].北京林业大学学报,2001,23(2):57-60,4.基金项目
“九五”国家科技攻关毛白杨子专题(96-011-02-04-02)资助 （96-011-02-04-02）
