厦门大学学报（自然科学版）2009，Vol.48Issue(4)：576-580,5.
河蚬乙酸乙酯提取物的抗氧化活性及其对人胆管癌细胞的抑制作用
Antioxidation Effect and Inhibition Effect on Human Cholangiocarcinoma Cells of Corbicula fluminea Ethyl Acetate Extract
摘要
Abstract
As a traditional chinese herb, Corbicula fluminea has many effects, such as, defervescence, curing the liver ailment, antialcoholism, and so on. We used fresh Corbicula fluminea body as the material and obtained the ethyl acetate extract by the method of ethyl acetate extraction. The ability of antioxidation was measured by DPPH method. The inhibition rate of cholangiocarcinoma cells was measured by MTT method. The morphological change of QBC939 cells was detected by ordinary inverted microscopy. The effect on cell cycles of QBC939 was observed by flow cytometry (FCM) assay. The results showed that ethyl acetate extract from Corbicula fluminea has strong ability to scavenging up DPPH radical, of which the value of IC50 was 0.6 mg/mL. And it could also inhibit the growth of QBC939 cells in a dose- and time-dependent manner, and the IC50 value was determined to be 250 μg/mL for 48 h treatment. The morphological of QBC939 cells changed significantly. At the same time, FCM showed that Corbicula fluminea ethyl acetate extract could change the cell cycle of QBC939 cells, and induce apoptosis. These results indicated that Corbicula fluminea ethyl acetate extract had the ability of antioxidation and effectively inhibited the proliferation of the human cholangiocarcinoma cell line QBC939 in vitro and acted as an apoptosis inducer in its antitumor effects via inducing morphological changes and cell cycle arrest. Thus, Corbicula fluminea ethyl acetate extract has the potential of becoming a natural drug, and it also provide a new thought and a new method for the therapy of cholangiocarcinoma.关键词
河蚬/抗氧化/胆管癌/增殖抑制/凋亡Key words
Corbicula fluminea/ antioxidation/ cholangiocarcinoma/ proliferation inhibit/ apoptosis分类
医药卫生引用本文复制引用
韩鹏,邱乒乒,李华亮,戴燕燕,李文岗,陈清西..河蚬乙酸乙酯提取物的抗氧化活性及其对人胆管癌细胞的抑制作用[J].厦门大学学报（自然科学版）,2009,48(4):576-580,5.基金项目
厦门市科技计划项目(3502Z20063021),厦门大学科技创新工程基金(XDKJCX20043001),福建省医学创新课题(2007-CXB-9)和厦门市卫生局资助项目(WSK0602) 资助 （3502Z20063021）
