中国农业科学(英文版)2003，Vol.2Issue(5)：578-582,5.
Cloning, Expression of apxI Gene of Actinobacillus pleuropneumoniae and Development of ELISA
Cloning, Expression of apxI Gene of Actinobacillus pleuropneumoniae and Development of ELISA
LIU Jian-jie 1HE Qi-gai 1CHEN Huan-chun 1WU Bin 1XU Xiao-juan 1LIU Jun-fa 1TANG Xian-chun 1BEI Wei-cheng1
作者信息
- 1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan,450070, P. R. China
- 折叠
摘要
Abstract
Based on the published nucleotide sequence of the apxICA of Actinobacillus pleuropneumoniaein Genbank(S4074), a pair of primers were designed. A 3 640 bp(4 687 -8 326 bp)gene fragment was ampli-fied by PCR from the isolated strain of A. pleuropneumoniae serovar 1. Then, it was cloned into pMD18-T,identified by both restriction endonuclease and sequence analysis, and inserted into pET-28a expression vectorto yield the expression plasmid. SDS-PAGE result indicated expression of apxICA in BL21 (DE3), Westernblot analysis showed the protein's immunogenicity. Using the expressed protein, ELISA was established to de-tect serum antibody against ApxI. The feature of ELISA to detect highly virulent A. pleuropneumoniae strainsinfection was proved by primary clinical application.关键词
Actinobacillus pleuropneumoniae/apxICA gene/Cloning/Prokaryotic expression/ELISAKey words
Actinobacillus pleuropneumoniae/apxICA gene/Cloning/Prokaryotic expression/ELISA分类
农业科技引用本文复制引用
LIU Jian-jie,HE Qi-gai,CHEN Huan-chun,WU Bin,XU Xiao-juan,LIU Jun-fa,TANG Xian-chun,BEI Wei-cheng..Cloning, Expression of apxI Gene of Actinobacillus pleuropneumoniae and Development of ELISA[J].中国农业科学(英文版),2003,2(5):578-582,5.
