摘要
Abstract
BACKGROUND: Neural stem cells (NSCs) can be ,separated into neurons, oligodendrocytes, astrocytes, etc. On the other hand, their separation, cultivation and identification need further experimental testimony.OBJECTIVE: To summarize recent research progress of survival, separation and identification of neuron stem cells in scientific study.RETRIEVAL STRATEGY: We searched the articles on the separation and culture of neuron stem cells via internet from PubMed from January 1999 to January 2005. The key words were "neuron stem cells, isolation, culture, differentiation" in English. There were 51 literatures in total. Inclusion criteria: The latest techniques on the separation and culture. Exclusion criteria: dupficated and inexact articles.LITERATURE EVALUATION: There were 25 articles on the study of techniques on separation and identification of neuron stem cells. Of those, 14 reviews and 11 thesis were included in this report.DATA SYNTHESIS: ① Separation and cultivation of NSCs: Flow cytometry, magnetic cell sortings (MACS) and primary culture were adopted. NSCs could be available straightly through flow cytometry, however, it was hard to spread such technology as the reagent and instrument were too expensive; MACS were easy operating and quite effective. It was suitable for NSCs transplantation due to the high purity. This makes MACS now quite practicable in NSCs separation; primary culture is now adopted by a majority of labs since the cost is acceptable. ② Cultivation and identification of NSCs: Single cells were separated from human fetal brain with parenzyme digestion, developed in vitro in FBS-free medium, added in bFGF and epidermal growth factor (EGF) which might activate cell growth and induce cells to differentiation via FBS. Then the cell nidogen antigen and mantre neural cells' antigen neurouspecific enolase (NSE) expressions were tested by immunoflueroseuce cytochemistry staining.CONCLUSION: Flow cytometry, MACS, and primary culture can be used to separate neuron stem cells, and the latter is the mostly adaptive approach. A single NSC forms a clone sphere v/a serf-reproduction It has been testified that expressing positive marks are NSCs by immunohistochemistry technology.关键词
神经干细胞/分离/分化/定向培养分类
医药卫生
