军事医学科学院院刊2001，Vol.25Issue(1)：5-8,4.
登革2型病毒43株NS1基因的克隆及在真核细胞中的表达
Cloning and expression of dengue 2 virus NS1 gene in eukaryotic cells
胡志君 1杨敬 1赵卫 1杨佩英 1秦鄂德 1范宝昌 1耿丽卿 1于曼1
作者信息
摘要
Abstract
Objective：To investigate the correct expression of dengue 2 virus 43 strain NS1 gene in transfected BHK-21 cell. Methods：The D2-43 DNA fragment coding for signal peptide plus NS1 protein was cloned between KpnⅠ site and EcoR Ⅰ site of expression plamid pcDNA3.1. The obtained recombinant vector pcDNA-NS1 was transfected into BHK-21 cells with electroporation technique. After selection by G418, resistant clones were screened by RT-PCR and Western blotting test. Results：The RT-PCR results of four in five randomly selected cell clones were positive. Western blotting test showed that NS1 gene could be expressed in BHK-21 cells. Conclusions：NS1 protein was capable of being expressed and appropriately processed in pcDNA-NS1 transfected BHK-21 cells. The present results suggest the feasibility of NS1-based DNA immunization.关键词
登革病毒/NS1蛋白/真核表达/印迹法,蛋白质分类
医药卫生引用本文复制引用
胡志君,杨敬,赵卫,杨佩英,秦鄂德,范宝昌,耿丽卿,于曼..登革2型病毒43株NS1基因的克隆及在真核细胞中的表达[J].军事医学科学院院刊,2001,25(1):5-8,4.
