动物营养学报2009，Vol.21Issue(4)：580-584,5.DOI:10.3969/j.issn.1006-267x.2009.04.025
壳聚糖对肉仔鸡血清花生四烯酸含量、磷脂酶A2活性及小肠胞内磷脂酶A2 mRNA表达的影响
Effects of Chitosan on Arachidonic Acid Content, Phospholipase A2 Activity in Serum and Expression of Cytosolic Phospholipase A2 mRNA in Small Intestine of Broilers
摘要
Abstract
The study was conducted to determine the effects of chitosan on content of arachidonic acid and the activity of phospholipase A2(PLA2) in serum, and relative expression of cytosolic phospholipase A2(cPLA2) mRNA in duodenum, jeju-num, and ileum of broilers.Two hundred and forty one-day-old Arbor Acre broilers were randomly allotted to six treatments with five replicates each and eight chickens in each replicate.Six groups were prepared for the study.Control group was the basal diet without chitosan.The other five groups were formulated by the addition of 50, 200, 500, 1 000 and 2 000 mg/kg of chitosan to the basal diet, respectively.The trial lasted for 42 days.The results showed that chitosan improved the con-tent of An, and the activity of PLA2 in serum, and also the expression of cPLA2 mRNA in duodenum, jejunum, and ileum of broilers in a quadratic dose-dependent manner (P<0.05), and the supplementation of 500 and 1 000 mg/kg chitosan to the diet were more effective.However, the supplementation of 2 000 mg/kg chitosan to diet was less effective.It was concluded that it was probably related to the improvement of PLA2 activity and the expression of cPLA2 mRNA in small intestine that chitosan may improve the immune function of broilers.关键词
壳聚糖/肉仔鸡/花生四烯酸/磷脂酶A2/胞内磷脂酶A2mRNAKey words
Chitosan/Broilers/Arachidonic acid/Phospholipase A2/Cytosolic phospholipase A2 mRNA分类
农业科技引用本文复制引用
李慧英,史彬林,闫素梅,冯永淼,郭晓宇..壳聚糖对肉仔鸡血清花生四烯酸含量、磷脂酶A2活性及小肠胞内磷脂酶A2 mRNA表达的影响[J].动物营养学报,2009,21(4):580-584,5.基金项目
国家自然科学基金项目(编号:30660130) （编号:30660130）
