厦门大学学报（自然科学版）2009，Vol.48Issue(4)：581-584,4.
L-半胱氨酸作为化妆品美白添加剂的作用机理
Study on the Mechanism of L-Cysteine as a Whitening Cosmetic Additive
摘要
Abstract
Tyrosinase(EC 1.14.18.1)is a key enzyme involved in the melanin biosynthesis.Nowadays,tyrosinase inhibition has become a hot spot on research of whitening cosmetic additive.In this paper,the effects of L-cysteine(L-Cys) on the activity of tyrosinase from both mushroom and mouse B16 melanoma cells were studied.The results showed that L-Cys could remarkably prolong the lag time and inhibit the activity of tyrosinase extracted from B16 cells,and the concentration for L-Cys leading to 50% tyrosinase activity lost (IC50) was 1.75 μmol/L and 15 μmol/L,respectively.Furthermore,the cells toxicity of L-Cys were assessed.The results showed that L-Cys has no obvious influence in cellular morphology,growth rate,metabolism of thiazole blue (MTT) of B16 melanoma cells,indicating that L-Cys is safe for using as a potential cosmetic additive.This research provided a basis for the usage of L-Cys as whitening cosmetic additive.关键词
L-半胱氨酸/黑色素/酪氨酸酶/B16黑色素瘤细胞Key words
L-cysteine/melanogenesis/tyrosinase/B16 melanoma cell分类
生物科学引用本文复制引用
杨美花,李智聪,刘凤娇,庄江兴,王勤,陈清西..L-半胱氨酸作为化妆品美白添加剂的作用机理[J].厦门大学学报（自然科学版）,2009,48(4):581-584,4.基金项目
福建省重点科技计划项目(2007N0051),福建省高等学校新世纪优秀人才支持计划项目资助 （2007N0051）
