中华医学杂志（英文版）2004，Vol.117Issue(4)：581-585,5.
IL-1α and ATP mRNA expression after ultraviolet irradiation in human keratinocyte original-SCC 12F cells
IL-1α and ATP mRNA expression after ultraviolet irradiation in human keratinocyte original-SCC 12F cells
骆丹 1闵玮 1吴迪 1苏忠兰1
作者信息
- 1. Department of Dermatology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
- 折叠
摘要
Abstract
Background Generally speaking, undifferentiated keratinocytes may synthesize a larger amount of IL-c and its production is decreased as cells complete differentiation gradually. Ultraviolet B (UVB)light can signigicantly stimulate the production and release of some cytokines. In this study we investigated the influence of UVB irradiation on IL-1 α and adenosine triphosphoric (ATP) mRNA expressions in the human keratinocyte (KC) of original squamous cell carcinoma line (SCC 12F cells).Methods The cultured SCC 12F cells were irradiated with 30 mJ/cm2 of UVB. Northern blot was employed to analyze the expression of IL-1 cα and ATP mRNA.Results There was a constitutive expression of IL-1α mRNA in SCC 12F cells. The expression increased in culturing time in regular KC medium and reached the highest expression at 120 hours.The expression level of IL-1 cα was up-regulated with two peaks at 6 hours and 72 hours, respectively after UVB irradiation. In comparison with IL-1 α mRNA expression, ATP mRNA was down-regulated,with similar biphasic peaks, compared with the sham irradiated group.Conclusions SCC12F cells may express IL-1α mRNA constitutively. After UVB irradiation, the mRNA expression of IL-1α and ATP will show opposite effect because of inflammation/immunity and energy consumption mechanisms.关键词
Interleukin-1/adenosine triphosphate/ultraviolet rays/keratinocytesKey words
Interleukin-1/adenosine triphosphate/ultraviolet rays/keratinocytes分类
医药卫生引用本文复制引用
骆丹,闵玮,吴迪,苏忠兰..IL-1α and ATP mRNA expression after ultraviolet irradiation in human keratinocyte original-SCC 12F cells[J].中华医学杂志（英文版）,2004,117(4):581-585,5.
