动物营养学报2009，Vol.21Issue(4)：585-591,7.DOI:10.3969/j.issn.1006-267x.2009.04.026
能量对蛋鸡卵巢促卵泡素受体mRNA表达及产蛋率的影响
Effects of Diet Energy on Expression of FSHR mRNA in Ovary and Laying Rates of Laying Hens
摘要
Abstract
This study was conducted to investigate the effects of diet energy on expression of FSHR mRNA in ovary during sexual maturity and laying rates of laying hens.Three hundred and sixty 8-week-old Hy-Line laying hens were divided into 5 groups with 4 replicates in each group and 18 hens in each replicate.Different energy level diets were confected under the same other nutrient levels.Reverse transcription polymerase chain reaction (RT-PCR) was used to determine the expression of FSHR mRNA in ovary of laying hens during sexual maturity.The results showed that expression of FSHR mRNA in ovary of laying hens of high energy groups increased from 14 to 18 week-old and decreased from 18 to 22 week-old.Expression of FSHR mRNA in ovary of laying hens of control and low energy groups increased with the increase of age.In 14 week-old, expression of FSHR mRNA in low energy Ⅱ group was significantly lower than those in other groups (P<0.05).In 18 week-old, expression of FSHR mRNA in control group was significantly higher than that in low energy Ⅱ group (P<0.05), no significant differences among other groups (low energy Ⅰ,Ⅱ, high energyⅠ,Ⅱ) were observed (P>0.05).In 22 week-old, no significant differences among control, high energy Ⅱ and low energy Ⅰgroups were observed (P>0.05), but their expression levels of FSHR mRNA were significantly higher than those in high energy Ⅰ and low energy Ⅱ groups (P<0.05).When the correlation between expression level of FSHR mRNA in ovary of 22 week-old laying hens and laying rates was analyzed, a very significant positive correlation for 22 and 28 week-old (P<0.01), a significant positive correla-tion for 35 week-old (P<0.05) and no significant correlation for 46 week-old (P>0.05) were obtained, respectively.The results suggested that proper energy level can increase expression of FSHR mRNA in ovary of laying hens and increase the reproductive performance.关键词
蛋鸡/能量/FSHRmRNA表达/产蛋率Key words
Laying hens, Energy/FSHR mRNA expression/Laying rate分类
农业科技引用本文复制引用
杨玉,黄应祥,曹果青,张桂贤,杨文平..能量对蛋鸡卵巢促卵泡素受体mRNA表达及产蛋率的影响[J].动物营养学报,2009,21(4):585-591,7.基金项目
山西省科技攻关项目(20080311033) （20080311033）
山西省高等学校科技项目(20080016) （20080016）
山西农业大学博士科研启动基金 （）
