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栗疫病菌泛素结合酶基因(CpUBC)全长cDNA的电子克隆

冯友军张会敏姜明国兰秀万

生物信息学2004，Vol.2Issue(2)：5-9,5.

栗疫病菌泛素结合酶基因(CpUBC)全长cDNA的电子克隆

In Silico cloning of full length cDNA of cryphonectria parasitica ubiquitin conjugated enzyme gene (CpUBC)

冯友军 ¹张会敏 ²姜明国 ³兰秀万⁴

作者信息

1. 广西大学,生物技术实验中心,广西, 南宁,530005
2. 广西大学,生物技术实验中心,广西, 南宁 ,530005
3. 广西民族学院,化学与生态学院,广西, 南宁,5300004
4. 广西医科大学,生物化学系,广西, 南宁, 530005
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摘要

Abstract

In silico cloning is a novel eukarytic gene cloning strategy, which is developed recently to achieve full length gene or cDNA, basing BLAST or alignment in Genbank of only partial target sequence information known to us, and the acquired cDNA sequence can be identified further through RT-PCR. Here we report a full length cDNA of ubiquitin conjugated enzyme gene (CpUBC) in chestnut blight fungi, Cryphonectria parasitica through in silica cloning, consisting of 1023 base pairs (bp) and predicted to contain a 444 bp of ORF starting with ATG initial codon, and stopping with TAG stop codon at the position of 245 and 686 nucleotide acid respectively, by the free software of ORF Finder provided by NCBI. Sequence analysis of the open coding region (ORF) of CpUBC, compared with those of Maganaporthe grisea, Neurospora crassa, and Metarhizium anisopliae, shows that CpUBC has 93.8%, 72.2%, 66.9% of identity in deductive amino acid level, in addition to 80.0%, 73.2%, 64.9% of identity in nucleotide acid level, respectively.

关键词

电子克隆/泛素结合酶/BLAST,Vector NTI

Key words

In silico cloning/Ubiquitin conjugated enzyme/BLAST/Vector NTI 

分类

农业科技

引用本文复制引用

冯友军,张会敏,姜明国,兰秀万..栗疫病菌泛素结合酶基因(CpUBC)全长cDNA的电子克隆[J].生物信息学,2004,2(2):5-9,5.

生物信息学

ISSN：1672-5565

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