首页|期刊导航|中国农业科学（英文版）|Characterization of Carboxylesterase Associated with Malathion Insensitivity in the Field Population of the Oriental Migratory Locust
中国农业科学（英文版）2008，Vol.7Issue(5)：591-598,8.
Characterization of Carboxylesterase Associated with Malathion Insensitivity in the Field Population of the Oriental Migratory Locust
Characterization of Carboxylesterase Associated with Malathion Insensitivity in the Field Population of the Oriental Migratory Locust
摘要
Abstract
Carboxylesterases (CarEs) from two field populations of the oriental migratory locust, Locusta migratoria manilensis (Meyen), were examined to try to understand their contribution to malathion insensitivity. The CarEs activities in Wudi population (WD) were 1.75- and 1.50-fold significantly higher than those in Huangliu population (HL) when α-naphthyl acetate (α-NA) and β-naphthyl acetate were used as substrates, respectively. Such elevated CarEs activities presented in the WD could be because of an increased staining intensity of the α-NA-hydrolyzing CarEs as shown on the nondenaturing polyacrylamide gel electrophoresis. Inhibition studies of CarEs using paraoxon and malaoxon indicated that CarE activities in the HL were more strongly inhibited than those in the WD. Furthermore, a 449-bp DNA fragment of Care was obtained from L. migratoria manilensis. Hemiquantity reverse transcription-polymerase chain reaction analysis showed that CarE gene expression level in the WD was higher than that in the HL. The higher CarE activities and the increased CarE mRNA level in the WD appeared to be associated with decreased susceptibility to malathion in the WD due to the application of organophosphorus insecticides.关键词
carboxylesterases, enzyme kinetics, enzyme inhibition, RT-PCR, Locusta migratoria manilensisKey words
carboxylesterases, enzyme kinetics, enzyme inhibition, RT-PCR, Locusta migratoria manilensis分类
农业科技引用本文复制引用
YANG Mei-ling,ZHANG Jian-zhen,ZHANG Jian-qin,GUO Ya-ping,MA En-bo..Characterization of Carboxylesterase Associated with Malathion Insensitivity in the Field Population of the Oriental Migratory Locust[J].中国农业科学（英文版）,2008,7(5):591-598,8.基金项目
This work was supported by the National Natural Science Foundation of China (30470219). （30470219）
