白求恩医科大学学报2000，Vol.26Issue(6)：592-594,3.
EGFP基因在前列腺癌PC-3M细胞系中的转染与表达
Transfection and expressiOn of green fluorescent protein gene in prostate carcinoma PC-3M cell line
邓宏 1常喜华 1代芊 2王娜 3刘建巨 1曹宏1
作者信息
- 1. 白求恩医大第三临床学院泌尿外科 吉林 长春130031
- 2. 第四军医大学 吉林军医学院
- 3. 长春市中心医院
- 折叠
摘要
Abstract
Objective：To reseach the expression of green fluorescent protein(GFP)gene in prostate car-cinoma PC-3M cell line and the efffect of cell cycle．Methods：GFP gene recombination DNA was construct-ed and PC-3M cells were transfected with lipofectin regant．The expression of GFP was observed under in-vert fluorescence microscope．Meanwhile，the cell cycle of positive cell colon was assayed by flOw cytome-ter．Resuits：After transfection，about 10％～15％cells expressed green fluorescent protein and there wasno significant change compared the cell cycle of positive cell colon with parents cells．Conclusion：GFP sup-plys a new way in reseaching metabolic phenomenon of live cells．关键词
绿色荧光蛋白/报告基因/脂质体/前列腺癌Key words
green fluorescent protein/reporter gene/lipofectin regant/prostate分类
生物科学引用本文复制引用
邓宏,常喜华,代芊,王娜,刘建巨,曹宏..EGFP基因在前列腺癌PC-3M细胞系中的转染与表达[J].白求恩医科大学学报,2000,26(6):592-594,3.
