中华医学杂志（英文版）2001，Vol.114Issue(6)：592-595,4.
瘦素对肝细胞葡萄糖氧化的急性与慢性作用
Effects of acute or prolonged exposure to leptin on hepatic glucose oxidation
曹筱佩 1傅祖植 1明文玉 1杨荣泽 1程桦1
作者信息
- 1. 中山医科大学附属第一医院内分泌科广州 510080
- 折叠
摘要
Abstract
Objective To observe the short-term and long-term effects of leptin on hepatic glucose oxidation and glucokinase gene expression.
Methods Rat hepatic cell line BRL was incubated with leptin of different doses (range from 10?ng/ml-200?ng/ml) for 1?h or 24?h. Glucose oxidation was determined by liquid scintillation counting. Glucokinase gene expression (corrected by β-actin) was determined by reverse transcription semi-quantitative polymerase chain reaction.
Results Treatment with leptin 10?ng/ml for 1?h had no significant effects on glucose oxidation in hepatic cells. However, at the doses ranging from 50?ng/ml to 200?ng/ml, leptin significantly inhibited glucose oxidation. These effects disappeared when the hepatic cells were exposed to leptin for 24?h. Glucokinase mRNA expression was reduced significantly after both 1?h and 24?h exposure to leptin (100?ng/ml) as compared to that of the control group.
Conclusion A low dose of leptin has no significant effect on glucose oxidation in hepatic cells. A relatively high dose of leptin has an acute inhibitory effect on the glucose oxidation in hepatic cells. This effect may likely involve the inhibition of glucokinase gene expression. The inhibitory effect on glucose oxidation is transient and disappears with prolonged exposure time.关键词
瘦素/糖代谢/肥胖症/肝细胞Key words
leptin/glucose metabolism/obesity/hepatic cell分类
医药卫生引用本文复制引用
曹筱佩,傅祖植,明文玉,杨荣泽,程桦..瘦素对肝细胞葡萄糖氧化的急性与慢性作用[J].中华医学杂志（英文版）,2001,114(6):592-595,4.
