动物学研究2008，Vol.29Issue(6)：592-598,7.DOI:10.3724/SP.J.1141.2008.06592
半滑舌鳎FTZ-F1 cDNA克隆及表达分析
Molecular Cloning and Expression Analysis of FTZ-F1 in the Half-smooth Tongue-sole, Cynoglossus semilaevis
摘要
Abstract
To investigate the expression characteristics of sex related gene of FTZ-F1 in the half-smooth tongue-sole (Cynoglossus semilaevis), the homoiogue FTZ-F1 (hsFTZ-F1) full-length cDNA was isolated from the testis by homologous cloning, and the cDNA included the open reading frame and a 66bp 5'-UTR, along with a 1619bp Y-UTR, encoding a predicted 485 amino acid protein. Sequence, tissue distribution and phylogenic analyses of the FTZ-F1 showed that the hsFTZ-F1 belonged to SF-1/Ad4BP group. The hsFTZ-F 1 transcripts were highly abundant in the gonads, kidneys, brain and head-kidneys, but weakly in other tissues. However, the expression level in the brain and head-kidney of female was highly abundant than in the male. The hsFTZ-F1 expression was highly abundant in the embryo than in the larvae, which suggested that the hsFTZ-F1 may be involved in the organogenesis in the tongue sole.关键词
半滑舌鳎/FTZ-F1/cDNA克隆/表达Key words
Cynoglossus semilaevis/FTZ-F1/cDNA cloning/Expression分类
生物科学引用本文复制引用
邓思平,陈松林,刘本伟..半滑舌鳎FTZ-F1 cDNA克隆及表达分析[J].动物学研究,2008,29(6):592-598,7.基金项目
Supported by grants from State 863 High-Technology R&D Project of China (2006AAIOA403) （2006AAIOA403）
Shandong Genetic Improvement Key Project for Agricultural Organism （）
Doctor Initial Funding of Guangdong Ocean University (0712103) （0712103）
