中国实用妇科与产科杂志2009，Vol.25Issue(8)：594-597,4.
凝血因子Ⅻ刺激外周血单核细胞对卵巢癌细胞侵袭能力的影响
Coagulation factor Ⅻ facilitate EOC cells invasion potential via stimulating 1L-8 secretion from monocytes
摘要
Abstract
Objective To explore the impact of monocytes (MOs) stimulated by F Ⅻ on the invasion potential of EOC. Methods Monocytes isolated from female healthy donors were culture and stimulated with Factor Ⅻ for 12 h. Tumor associated macrophage (TAM) were derived from ascites of EOC. India ink engulfing test was used to detect phagocytic capacity of MOs stimulated by F Ⅻ. Invasive potential of EOC, including ES-2, SKOV-3, HO-8910 cells were investigated by Matrigel invasion assay. The signaling pathway involved in MOs activated by F Ⅻ were measured by Transfactor family Kits, including AP-1 ,STAT, Inflammation 1 and Oncogene 3. Results (1) The percentage of engulfing Indian ink were 0.66 s0.04 in TAM and O. 36 s0.03 in monoeytes stimulated by FⅫ, which were siguificanfly higher than that mono-cytes from healthy donors(0.27±0.018) (P < 0.01 and P = 0.033). (2) ES-2 cells were shown more invasive cell number when the supematant from MOs+ Ⅻ was used as chemo-attractant (110.9±5.046), fewer than that of ascites group (157.5±14.86), but significantly more than that of negative control group in which cells were cultured with usualmedium (39.14±10. 91). When adding Centuximab into supernatants, decreased invasive cell numbers (42.43±9.097) were observed. Conclusion FⅫ might educate MOs differentiation toward TAM like cells, thus accelerated me-tastasis and invasion of EOC in the peritoneum.关键词
卵巢肿瘤/单核细胞/凝血因子Ⅻ/肿瘤相关巨噬细胞/白介素-8Key words
ovarian cancer/monocyte/coagulation factor Ⅻ/tumor associated macrophage/IL-8分类
医药卫生引用本文复制引用
王睿黎,张婷,马政文,王颖,狄文,徐红,马庆良,程忠平,汪希鹏..凝血因子Ⅻ刺激外周血单核细胞对卵巢癌细胞侵袭能力的影响[J].中国实用妇科与产科杂志,2009,25(8):594-597,4.基金项目
国家自然基金资助项目(30600672) （30600672）
上海市科委启明星后跟踪计划(06QH14010) （06QH14010）
