摘要
Abstract
Two DNA fragments,promoter(fragment 1)and promoter with signal peptidecoding sequence (fragment 2) of sporamin from sweet potato Nahshu 88,a widely cultivated variety in China,were amplified by the polymerase chain reaction (PCR).The amplified fragments were cloned and then sequenced. Sequence comparison showed that the fragment 1 sequence was almost identical to the corresponding region of sporamin gene, gSPO-A1, but different from that of gSPOR5-31.The sequence of fragment 2,including some cis regulatory elements,was different from the corresponding region of gSPO-A1 and gSPOR5-31.Comparison of signal peptide-coding sequence among fragment 2,gSPO-A1 and gSPOR5-31 showed 8 nucleotides changed,which appeared in the region of pre-segment, not in the region of pro-segment.These results indicated that the 5’-fianking region of sporamin genes was much conserved, but there was some variation,especially in the promoter region,which maybe play some role in gene expression.关键词
甘薯/贮藏蛋白基因/启动子/信号肽编码区/PCR/序列分析Key words
Ipomoea batatas/Sporamin gene/Promoter/Signal peptide-coding region/PCR/Sequence analysis分类
农业科技
