中国人兽共患病杂志2001，Vol.17Issue(2)：59-61,34,4.
程氏东毕吸虫成虫体表扫描电镜观察
SCANNING ELECTRON MICROSCOPY OF INTEGUMENTAL SURFACE OF ADULT ORIENTOBILHARZIA CHENI
王春仁 1张广军 2王文君 3许成彬 3于万才 1建华 1许腊梅1
作者信息
- 1. 黑龙江省兽医科学研究所
- 2. 中国预防医学科学院寄生虫病研究所
- 3. 大庆市兽医卫生防疫站
- 折叠
摘要
Abstract
Aim To characterize the integumental surface of adult O. cheni by scanning electron microscopy at 650 to 8000 magnifications. Method The adult worms were recovered form naturally infected sheep, then fixed in 2.5 % glutaraldehyde. The
specimens were washed in phosphate buffer 3 times and postfixed in 1% osmium tetroxide for 1 hour, then they were dehydrated in
graded series of ethanol and dried using CO2 as transitional fluid. Finally the specimens were mounted onto aluminum stubs and coated
with carbon gold and observed with Hitachi JSM - 800. Results There are thousands of tegumental folds and sensory bulbs on the
surface of adult male, but surface of the female' anterior and middle part is comparatively smooth; the posterior part bears sensory bulbs and spine, but no tegumental large mass with a hole. Conclusion The adult male shares many commen futures just like tegumental folds, sensory bulbs etc with O. turkestanica, but the distribution and quantity of the tegumental folds and sensory bulbs are different from each other. The female posterior part bears sensory bulbs and spine, but no tegumental large mass with a hole which is
a special character of O. turkestanica. All of these support that O, cheni is a distinctive species.关键词
程氏东毕吸虫/SEM/体表显微结构观察分类
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王春仁,张广军,王文君,许成彬,于万才,建华,许腊梅..程氏东毕吸虫成虫体表扫描电镜观察[J].中国人兽共患病杂志,2001,17(2):59-61,34,4.
