农业生物技术学报2005，Vol.13Issue(5)：596-600,5.
用DNA差异显示技术分离、测序和鉴定猪的两个新分子标记及其与性状的关联分析
Isolation, Sequencing and Identification of Two Pig Novel Molecular Markers Using DNA Differential Display Technique and Their Trait Analysis
摘要
Abstract
In order to find more candidate genes or DNA molecular markers which are correlated with the traits, we used DNA differential display technique to scan the whole genome of F2 pig population based on Large White xMeishan, and two molecular markers were found to be significantly correlated with the traits. Marker 1 was associated with: estimated backfat thickness at last rib (P＜ 0.01), estimated backfat thickness at last 3～4th rib(P＜ 0.01), estimated lean meat percentage (P＜ 0.01), bone weight (P ＜ 0.05),bone percentage (P ＜ 0.05), fat meat weight (P ＜ 0.05), fat meat percentage (P ＜ 0.05), ratio of lean meat to fat meat (P ＜ 0.05), backfat thickness at shoulder (P ＜ 0.05), backfat thickness at 6-7th thorax (P ＜ 0.01), and marker 2 was associated with: longissimus dorsi pH (P＜ 0.05), drip loss rate (P ＜ 0.05), water holding capacity (P ＜ 0.05), meat color score oflongissimus dorsi (P ＜ 0.05), water moisture of longissimus dorsi (P ＜ 0.05), rib numbers (P ＜ 0.05), bone percentage (P ＜ 0.05), backfat thickness at thorax-waist (P ＜ 0.05),gaster net weight (P ＜ 0.01). The two markers were then isolated, sequenced and identified. BLAST analysis revealed that the two markers were not homologous to any of the known porcine genes from GenBank and subsequenfiy submitted to GenBank database(Accession number: CN605659 and AY626262). The method used in this study provides an additional useful tool in the investigation of candidate genes or DNA molecular markers, which were correlated with the traits.关键词
猪/DNA差异显示/分子标记/关联分析Key words
pig/DNA differential display/molecular marker/association analysis分类
农业科技引用本文复制引用
刘永刚,熊远著,邓昌炎,雷明刚,左波,李家连,蒋思文,李凤娥,郑嵘..用DNA差异显示技术分离、测序和鉴定猪的两个新分子标记及其与性状的关联分析[J].农业生物技术学报,2005,13(5):596-600,5.基金项目
The Seate Key Basic Research and Development plan (973) Project (No. G2000016105) of China （973）
