中国临床康复2004，Vol.8Issue(27)：5974-5976,3.
强直性脊柱炎与肿瘤坏死因子基因启动子-308位点多态性相关的假设验证
Hypothetic verification of the correlation between polymorphism in the promoter region-308 of the tumor necrosis factor gene and ankylosing spondylitis
摘要
Abstract
BACKGROUND:The positive rate of human leucocyte antigen(HLA)-B27 in the patient with ankylosing spondylitis(AS) has significant differences among different reports,which might be induced by environment but also could be affected by other HLA or non-HIL factors.It is presumed that some other factors might play certain roles in the genesis of AS. OBJECTIVE:To investigate the correlation between the polymorphism in the promoter region of tumor necrosis factor(TNF α ) and AS. DESIGN:A non-randomised controlled trial. SETTING and PARTICIPANTS:Data collection setting:United Hospital Affiliated to Tongji Medical University.Participants:Totally 78 AS cases were selected from Wuhan Region during June 2000 to January 2003,who were in accordance with diagnostic criteria modified by Van der Linder in 1984,including 67 males and 11 females,of which the mean age was(37.4± 12.8) years old.52 HLA-B27 positive healthy Wuhan citizens,who were the Han nationality were selected in control group, which included 44 male and 8 female subjects with a mean age of(39.2± 14.4) years old. INTERVENTIONS:A Nco I restrictive site polymorphism in the promoter region of the TNF gene was detected by polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP) in both 78 patients with AS and 52 HLA-B27 positive healthy controls.Meanwhile,enzyme linked immunosorbent assay(ELISA) was used to assay serum TNF α level in both patients and controls. MAIN OUTCOME MEASURES:The relationship between the genotype of -308 promoter region of TNF gene and AS. RESULTS:The -308.1.1 genotype was found in 61/78(78.2% ) AS patients,which was significantly higher compared with 29/52(55.8% ) of HLA-B27 positive healthy subjects(χ 2=6.8,P< 0.05).The -308.1.2 and -308.2.2 genotypes in AS patients were 20% and 1% respectively,which were significantly lower than 38% (χ 2=9.3,P< 0.05) and 5.8% (χ 2=11.4,P< 0.01).The levels of serum TNF α [(15.80± 11.20) and 28.00 μ g/L] and C reactive protein[(44.1± 26.3) and 105.3 ng/L],erythrocyte sedimentation rate(ESR)[(27.7± 11.5) and 61.5 mm per hour] and immunoglobulin A[(33.5± 19.7) and 29.2 g/L] in the AS patients with the latter two genotypes were significantly higher than that of AS patients with -308.1.1 genotype(t=3.5,2.1,3.1,P< 0.05- 0.01). CONCLUSION:The -308.1 allele is more common in patients with AS than in HLA-B27 positive normal controls,which implies that the polymorphism in this region of TNF gene is correlated with AS.关键词
脊柱炎,强直性/肿瘤坏死因子/启动区(遗传学)/多态现象(遗传学)分类
医药卫生引用本文复制引用
刘巧红,吴清敏,滕云,沈凌汛,王慧,陈燕,杨真荣..强直性脊柱炎与肿瘤坏死因子基因启动子-308位点多态性相关的假设验证[J].中国临床康复,2004,8(27):5974-5976,3.基金项目
湖北省卫生厅第五个三年医药卫生科研资助项目( WJ01524) （ WJ01524）
