动物学研究2008，Vol.29Issue(6)：603-607,5.DOI:10.3724/SP.J.1141.2008.06603
兔骨形态发生蛋白15 cDNA部分序列的克隆及其在卵母细胞中的表达
Cloning of Rabbit Bone Morphogenetic Protein 15 and Its Expression During in vitro Maturation of Rabbit Oocytes
尹萍 1季金强 1李霖 1丁家桐1
作者信息
- 1. 扬州大学动物科学与技术学院,扬州,225009
- 折叠
摘要
Abstract
Partial cDNA sequence of rabbit BMP15 was cloned by RT-PCR from rabbit ovaries, showing a similarity of 83%-90% with the BMPI5 nucleotide sequences in humans, mice, ovine, sheep, cows and pigs. The expression of BMPI5 in rabbit cumulus-oocyte complexs during oocytes in vitro maturation (IVM) was measured by fluorescent quantitative RT-PCR method. BMP 15 was expressed at low levels in immature oocytes and increased to the highest level at 16h of IVM, which coincides with the time of cumulus cell expansion, then declined slowly under IVM cultivation. The expression pattern of BMP15 suggested that it might be important in cumulus expansion in rabbits.关键词
兔/骨形态发生蛋白15/卵母细胞/克隆/荧光定量RT-PCRKey words
Rabbit/Bone morphogenetic protein 15/Oocyte/Gene cloning/Fluorescent quantitative RT-PCR分类
生物科学引用本文复制引用
尹萍,季金强,李霖,丁家桐..兔骨形态发生蛋白15 cDNA部分序列的克隆及其在卵母细胞中的表达[J].动物学研究,2008,29(6):603-607,5.
