中山大学学报(自然科学版)2002，Vol.41Issue(3)：60-63,4.
水稻小GTP蛋白OsRab5A的表达、纯化和GTP结合分析
Prokaryotic Expression, Protein Purification and GTP-binding Assay of Rice rab5A Gene
摘要
Abstract
Osrab5A, a rab5A gene cloned from Rice (Oryza sativa L.), encodes a putative Rab protein with high similarity to mammalian Rab5A, which was localized on the early endosome and in the cytoplast. Rab5A was identified in mammalian cells as a rate-limiting factor for homotypic endosome fusion. The complete coding sequences of Osrab5A cDNA clone were amplified and inserted into the prokaryotic expression vector pGEX4T1. The resulting recombinant plasmid with correct reading frame, pG-5AE, was introduced into E.coli. BL21 (DE3),The GST tagged fusion protein, GST-OsRab5A was induced by IPTG induction and purified on a GSTrapTM column. The GTP-binding assay of this protein showed the GST tagged OsRab5A protein has GTP-binding ability in vitro.关键词
水稻/Rab蛋白/GTP结合Key words
rice (Oryza sativa L.)/Rab protein/GTP-binding assay分类
生物科学引用本文复制引用
刘筱斌,林慧贤,梁承邺,刘良式..水稻小GTP蛋白OsRab5A的表达、纯化和GTP结合分析[J].中山大学学报(自然科学版),2002,41(3):60-63,4.基金项目
国家转基因植物研究与产业化专项资助项目(J00-A-009) （J00-A-009）
中科院华南植物所所长基金项目(10-00-312) （10-00-312）
