首页|期刊导航|高技术通讯（英文版）|Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment
高技术通讯（英文版）2003，Vol.9Issue(1)：6-10,5.
Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment
Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment
摘要
Abstract
In order to improve the frequency of indica rice transformation by biolistic bombardment, suitable culture conditions for embryonic calli,an optimal selection scheme for resistant calli and seedlings, and optimum bombardment parameters a investigated by using 14 commercially important indica rice cultivars. The main results show that the CC medium with 36g/L mannitol is a scheme subculture medium in which the browning of indica rice calli can be mitigated significantly; The concentration of 30～40mg/L Hyg or 150～200mg/L G418 or 10～20 mg/L Basta is suitable for selection of resistant calli; The transformation parameters of 100μg gold powder absorbing 0.2μg DNA per shot and 900 psi helium pressure and 6 cm bombardment distance and bombarded twice for each plate give the best result; Keeping the target calli on osmotic medium containing 60g/L mannitol from 12 ～24h before bombardment to 24～48h after it can increase the efficiencies of transformation . Furthermore, some transgenic indica rice plants are obtained using this optimized transformation system.关键词
indica rice/biolistic bombardment/transformation/callusKey words
indica rice/biolistic bombardment/transformation/callus分类
农业科技引用本文复制引用
..Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment[J].高技术通讯（英文版）,2003,9(1):6-10,5.基金项目
The project supported by the Chinese 863 High-Technology Program (No.863-101-01-01-01) and Chinese Post-Doctoral Science Foundation (2001-14) （No.863-101-01-01-01）
