林产化学与工业2008，Vol.28Issue(2)：6-10,5.
高速逆流色谱分离制备胡椒中的胡椒碱
Preparative Separation and Purification of Piperine from Piper nigrum Linn.by High-speed Counter-current Chromatography
摘要
Abstract
Piperine,a major plant alkaloid presenting in pepper,was successfully separated and purified from the crude extract of Piper nigrum Linn.by high-speed counter-current chromatography(HSCCC)with a two-phase solvent system composed of petroleum ether(60-90℃)-ethyl acetate-methanol-water(1:0.8:1:0.8,volume ratio).The upper phase was used as stationary phase,while the lower phase was used as mobile phase.From 300 mg of crude extract,65.2 rag of piperine was obtained at 99.1% purity in one-step separation.The structure of the target compound was identified by EI-MS,1H NMR and 13C NMR.关键词
胡椒/胡椒碱/高速逆流色谱/分离纯化Key words
Piper nigrum Linn./piperine/high-speed counter-current chromatography/separation and purification分类
化学化工引用本文复制引用
王晓,苑金鹏,耿岩玲,李福伟,王岱杰,刘建华..高速逆流色谱分离制备胡椒中的胡椒碱[J].林产化学与工业,2008,28(2):6-10,5.基金项目
山东省重大科技专项计划(2006GG1109078,1108097-05) （2006GG1109078,1108097-05）
山东农业大学作物生物学国家重点实验室开放基金(2005-04) （2005-04）
