中华医学杂志（英文版）2002，Vol.115Issue(4)：614-616,3.
硫酸多糖916对细胞因子和H2O2诱导ECV304细胞产生一氧化氮的影响
Effect of polysaccharide sulfate 916 on the production of nitric oxide in ECV304 cells induced by cytokines and H2O2
摘要
Abstract
Objective To investigate the effect of polysaccharide sulfate 916 (PS916) on the production of nitric oxide (NO) in ECV304 cells induced by tumor necrosis factor-α (TNFα), interleukin-1β (IL-1β) and H2O2 in vitro. Methods Production of NO in ECV304 cells was measured by the Griess method and the proliferation of cells was tested by the MTT method. The activity of NO synthase was detected spectrophotometrically. Results Production of NO in ECV304 cells decreased after treatment with 40ng/ml IL-1β and 40ng/ml TNFα, but increased in the presence of H2O2 0.1mmol/L. PS916 significantly enhanced NO production in ECV304 cells in a dose-dependent manner in the TNFα and IL-1β treated groups and decreased it in the H2O2 treated group. Proliferation of ECV304 cells was inhibited by TNFα and H2O2 and no effect was found in the IL-1β treated group. PS916 increased the proliferation of cells treated with TNFα and H2O2 dose-dependently. In vitro, PS916 has no effect on the activity of NO synthase.Conclusion PS916 has a protective effect on ECV304 cells exposed to IL-1β, TNFα and H2O2.关键词
ECV304 硫酸多糖916 一氧化氮 H2O2Key words
ECV304 cells/polysaccharide sulfate 916/nitric oxide/cytokines分类
医药卫生引用本文复制引用
任德成,耿美玉,杜冠华..硫酸多糖916对细胞因子和H2O2诱导ECV304细胞产生一氧化氮的影响[J].中华医学杂志（英文版）,2002,115(4):614-616,3.基金项目
This study was supported by the National Natural Science Foundation of China (No. 3001161940). （No. 3001161940）
