中国临床药理学与治疗学2008，Vol.13Issue(6)：614-619,6.
异丙酚对大鼠脊髓背角神经元TTX敏感型钠通道失活和去失活效应的影响
Effects of propofol on TTX-sensitive voltage-gated sodium channels in rat acutely dissociated spinal dorsal horn neurons
摘要
Abstract
AIM:To investigate the effects of propofol on TTX-sensitive voltage-gated sodium currents and its mechanism in acutely dissociated rat spinal dorsal horn neurons. METHODS:Seven-day-old male SD rats were decapitated, a segment of lumbar enlargement of the spinal cord was dissected out, then the dorsal horn neurons were dissociated, and the whole-cell patch clamp recording were performed. Propofol of 0.3-30 μmol/L was applied 100 μm apart from the cells. The currents were activated by a 30 ms-pulse of-10 mV from holding petential(-80 mV). The inhibitory concentration in 50% of the sodium current amplitude (IC50) value of propofol was estimated. For investigation of sodium channels inactivation, propofol of IC50 level was applied, sodium currents were activated by a 30 ms-pulse of 0 mV after a 30 ms-prepulse from -80 mV to -20 mV with a voltage step of 10 mV. For investigation of sodium channels deinactivation, sodium currents were activated by a 30 ms-pulse to -10 mV after a 30 ms-prepulse to -10 mV from holding pontential (-80 mV) under propofol of IC50 level, the intervals between the two pulses were from 2 ms to 24 ms. RESULTS:Propofol could inhibit tetrodotoxin-sensitive sodium currents in a concentration-dependent manner, the IC50 value of propofol was (5.35±0.25) μmol/L. In inactivating sodium channels test, from -60 mV to -40 mV of the prepulse, propofol of IC50 level could suppress sodium currents activated by the test pulse significantly (P<0.05); in deinactivating sodium channels test, the currents activated by the test pulse with the intervals from 2 ms to 6 ms were significantly suppressed by propofol with IC50 level (P<0.01, P<0.05). CONCLUSION:Propofol inhibited TTX-sensitive voltage-gated sodium currents in rat dorsal horn neurons, which was mediated by facilitating sodium channels inactivating and inhibiting sodium channels from its deinactivation.关键词
异丙酚/背角神经元/钠电流Key words
propofol/dorsal horn neuron/sodium current分类
医药卫生引用本文复制引用
刘红亮,郭伟韬,曾因明,姚尚龙..异丙酚对大鼠脊髓背角神经元TTX敏感型钠通道失活和去失活效应的影响[J].中国临床药理学与治疗学,2008,13(6):614-619,6.基金项目
This project was supported by the fund of Jiangsu provincial key laboratory of Anesthesiology(KJS06004) （KJS06004）
