华东师范大学学报（自然科学版）Issue(3)：61-68,8.
中华绒螯蟹(Eriocheir japonica sinensis)内转录间隔区1(ITS1)大小变异的研究
Study on Size Variation of rDNA Internal Transcribed Spacer 1 in Eriocheir japonica sinensis (Decapoda, Grapsidae)
摘要
Abstract
The intra-individual size variation of rDNA ITS1 (first internal transcribed spacer ofrDNA) was detected in some invertebrates, such as Schistoaoma japonicum, Paragonimus west-ermani, Simulium damnosum. In our experiment of investigating the primary structure of ITS1of Eriocheir japonica. sinensis the intra-individual size polymorphism was determined at first,but further study showed that the polymorphism was an artifact, which was due to PCR primersmismatching. This conclusion was finally demonstrated by verifying conditions of PCR and re-amplifying the ITS1 domain using redesigning primer that was corresponding to 5.8S rDNA se-quences of E. japonica. sinensis that obtained in this study. The nucleotide sequence of the ITS1of E. japonica sinensis has been firstly determined in this species.关键词
中华绒鳌蟹/ITS1/大小变异/十足目Key words
Eriocheir japonica sinensis/ITS1/size variation/decapoda分类
生物科学引用本文复制引用
唐伯平,周开亚,宋大祥,陈立侨..中华绒螯蟹(Eriocheir japonica sinensis)内转录间隔区1(ITS1)大小变异的研究[J].华东师范大学学报（自然科学版）,2003,(3):61-68,8.基金项目
国家自然科学基金面上项目(30170116),国家自然科学基金重点项目(30130040),江苏省教育厅自然科学基金项目(KJD180005) （30170116）
