中国人兽共患病学报2009，Vol.25Issue(7)：619-622,4.
狂犬病毒新型快速荧光斑点抑制实验方法的建立
A novel rapid fluorescence focus inhibition test for the detection of rabies virus antibody
摘要
Abstract
The objective of the present study was to establish a novel rapid fluorescence focus inhibition test (RFFIT GFP)for the detection of rabies virus antibody, in which a chimeric rabies virus expressing green fluorescent protein (HEP GFP) was used as the basic virus strain in RFFIT GFP assay, and a few serum samples from human, dog and cat were detected by this new method .The optimal serum dilution, virus dosage and infection time were determined in 24 serum samples from human, dog and cat by using RFFIT GFP, RFFIT and ELISA assays. The result showed that these 3 methods gave a good consistency. But RFFIT GFP was found to be more convenient and economic for the detection of rabies virus antibody.关键词
狂犬病毒/绿色荧光蛋白/快速荧光斑点抑制实验Key words
rabies virus/green fluorescent protein/rapid fluorescent focus inhibition test分类
农业科技引用本文复制引用
梁红茹,刘晓慧,陈晶,孙招金,郭霄峰..狂犬病毒新型快速荧光斑点抑制实验方法的建立[J].中国人兽共患病学报,2009,25(7):619-622,4.基金项目
国家自然科学基金资助项目(30871876),教育部长江学者和创新团队发展计划(IRT0723),广东省自然科学基金团队项目(5200638) （30871876）
