中国组织工程研究与临床康复2009，Vol.13Issue(31)：6197-6200,4.DOI:10.3969/j.issn.1673-8225.2009.31.046
肝脏射频消融对大鼠外周血单个核细胞OX62,OX6及CD86表达的影响
Effects of radiofrequency ablation on 0X62,0X6 and CD86 expression in peripheral blood mononuclear cells of the rat liver
摘要
Abstract
BACKROUND:Previous studies have confirmed that radiofrequency ablation(RFA)has not only efficiently killed tumor cells,but also improved immune suppression of organism.Antigen presenting cells play an important role during anti-tumor immune reaction.Dendritic cells are most powerful antigen presenting cells.OBJECTIVE:To study the influence of RFA on OX-62,OX-6 and CD86 expression of rat peripheral blood mononuclear cells.DESIGN,TIME AND SETTING:The randomized controlled animal observation was performed at the School of Oncology of Peking University from June 2005 to March 2006.MATERIALS:A total of 12 Sprague Dawley rats were equally and randomly assigned into control and 1 -week radiofrequency groups.METHODS:2 mL peripheral blood was extracted from rats of the control group following rats were sacrificed.The left lobe of rat liver was exposed,inserted with acicular electrodes at tilted position.The acicular electrodes were spread out.The action time was 4 minutes.After RFA,the rats were given anti-infective therapy.2 mL peripheral blood was taken out after they were put to death 1 week after RFA.MAIN OUTCOME MEASURES:The following parameters were measured:pathomorphological observation on the liver of normal rats after RFA;OX-62,OX-6 and CD86 expression of peripheral blood mononuclear cells before and after RFA.RESULTS:Pathologyical examination after RFA showed the characteristic that coagulation necrosis and cellular degeneration and granulation tissue forming appeared from target center to peripheral of the target.Positive expression rate of OX-62 in rat peripheral blood in the 1-week radiofrequency group[(0.70±0.16)%]was significantly higher than in the control group [(0.34±0.08)%,P ＜0.05].No significant difference in positive expression rate of OX-6 and CD86 in the peripheral blood mononuclear cells between both groups(P＞0.05).CONCLUSION:RFA can promote the increased number of precursor Dendritic cells in rat peripheral blood,which may be contributed to improve the ability to angtigen presentation during immune response.Dai Wei-de,Doctor,Associate chief physician,Department of Ultrasound,Beijing Hospital of Ministry of Public Health,Beijing 100730,China dai.weide@126.com关键词
射频消融/大鼠/单个核细胞分类
基础医学引用本文复制引用
戴维德,范智慧,陈敏华,河福金,李洪民,刘静贤..肝脏射频消融对大鼠外周血单个核细胞OX62,OX6及CD86表达的影响[J].中国组织工程研究与临床康复,2009,13(31):6197-6200,4.基金项目
Supported by:the Key Raising Special Program of Science and Technology Committee of Beijing City,No.Z0005190040431,北京市科委重大项目培育专项(Z0005190040431) （Z0005190040431）
