中国组织工程研究与临床康复2008，Vol.12Issue(32)：6201-6208,8.
Collagen type Ⅲ is an important linking molecule between generated renal tubules and an artificial polyester interstitium Reticulin as a linker between generated tubules and the interstitium
Collagen type Ⅲ is an important linking molecule between generated renal tubules and an artificial polyester interstitium Reticulin as a linker between generated tubules and the interstitium
W.W.Minuth 1L.Denk 1A.Blattmann 1H.Castrop2
作者信息
- 1. Department of Molecular and Cellular Anatomy,University Street 31,D-93053 Regensburg,Germany
- 2. Department of Physiology,University of Regensburg,University Street 31,D-93053 Regensburg,Germaay
- 折叠
摘要
Abstract
In the field of regenerative medicine,much consideration has been given to stem/progenitor cells for the future treatment of acute and chronic renal failure.For this strategy to be effective,however,cell biological information about tubule development within the diseased organ is needed.Unresolved cell-biological issues relating to this kind of treatment include①the integration of stem/progenitor cells,②their differentiation into site-specific cell types,and③the spatial formation of new tubules.To better understand the mechanisms related to this technology,renal tubules were generated at the interphase of an artificial interstitium by using advanced culture techniques.Stern/progenitor cells derived from neonatal rabbit kidney were covered with layers of polyester fleece,placed in a perfusion culture container,and superfused for 13 days with fresh and chemically defined Iscove's Modified Dulbeccos Medium(IMDM) containing aldosterone (1×10-7mol/L).The spatial growth of tubules was registered by scanning electron microscopy(SEM) and on whole mounts or cryosections labeled with soybean agglutinin,silver stain and monoclonal antibodies reacting with collagen type Ⅲor laminin γ1.SEM revealed that the generated tubules were completely covered by a basal lamina.The lamina fibroreticularis exhibited numerous fibers connecting the basal aspect of generated tubules with the surrounding polyester fibers.Cryosections labeled with monoclonal antibodies anti-collagen type Ⅲ and silver stain demonstrated the formation of numerous fibers spanning between the basal lamina of generated tubules and neighboring polyester fibers.In matured kidney tubules the samg arrangement of collagen type Ⅲ fibers is observed as that for generated tubules.This work shows that collagen type Ⅲ is a relevant molecular linker between the basal aspect of generated renal tubules and the polyester fibers of the artificial interstitium.关键词
组织工程学/灌注培养/干/祖细胞/肾/小管/人工间质/基底膜/Ⅲ型胶原/聚酯Key words
tissue engineering/perfusion culture/stem/progenitor cells/kidney/tubule/artificial interstitium/basal lamina/collagen type Ⅲ/polyester分类
医药卫生引用本文复制引用
W.W.Minuth,L.Denk,A.Blattmann,H.Castrop..Collagen type Ⅲ is an important linking molecule between generated renal tubules and an artificial polyester interstitium Reticulin as a linker between generated tubules and the interstitium[J].中国组织工程研究与临床康复,2008,12(32):6201-6208,8.
