国际眼科杂志2005，Vol.5Issue(4)：621-624,4.
PTEN mRNA在兔晶状体上皮细胞增殖中的表达
PTEN mRNA expression in proliferative LECs of the rabbit
张瑞君 1张劲松1
作者信息
- 1. 110001,中国辽宁省沈阳市,中国医科大学附属第一临床学院眼科
- 折叠
摘要
Abstract
· AIM: To observe the effects of PTEN and the tumor suppressor gene in proliferative LECs of the rabbit.·METHODS:Forty-two white rabbits were randomly divided into test group (36 rabbits) and control group (6rabbits). The transparent lenses of treated rabbits were operated with extra capsular cortex extraction, and the controls were kept untouched. The rabbits were sacririced at 1d, 3d, 1wk, 2wK, 1mo and 2mo after surgery.Immunohistochemistry was applied to detect proliferative cellular nuclei antigen (PCNA) as well as hybridization in situ and reverse-transcriptase polymerase chain reactions (RT-PCR) were applied to detect phosphatase as well as tensin homology deleted on chromosome 10(PTEN) mRNA in lens equartor.·RESULTS:PCNA expression increased significantly to a high level at 1wk, began to reduce 2wk later, and recovered to the normal level 1 or 2mo after the surgery.PTEN mRNA expressed positively in normal rabbit LECs.The relative PTEN mRNA contents reduced greatly at 1dafter operation and remained low level at 3d. It began to increase slightly at 1wk, kept rising at 2wk and regained the normal expression after 1 or 2mo. There was inverse correlation between the PTEN mRNA and PCNA expression.·CONCLUSION: PTEN mRNA expresses positively in normal rabbit LECs plasma. PTEN participates in LECs proliferation and correlates with lens proliferative conditions.关键词
晶状体/上皮细胞/PTENKey words
crystalline/lens/epithelial/animal/experiment/PTEN分类
医药卫生引用本文复制引用
张瑞君,张劲松..PTEN mRNA在兔晶状体上皮细胞增殖中的表达[J].国际眼科杂志,2005,5(4):621-624,4.
