中国农业科学（英文版）2006，Vol.5Issue(8)：622-628,7.
The Herbicidal Activity of Mutant Isolates from Botrytis cinerea
The Herbicidal Activity of Mutant Isolates from Botrytis cinerea
摘要
Abstract
Fifteen mutant isolates were obtained by ultraviolet mutation from parent isolate Botrytis cinerea BC-4. Among them three mutant isolates, BC4-1, BC4-2, and BC4-15, showed strong herbicidal activity. BC4-1 showed maximum herbicidal activity for inhibition of germination and growth of Digitaria sanguinalis L. and Amaranthus retroflexus L. The results also showed that herbicidal activity was influenced by differing pH of PD media, with pH value of 4.0 being the optimum.The crude toxin was extracted using chloroform, petroleum ether, and ethyl acetate, respectively, and the ethyl acetate extracts showed the strongest inhibitory activity on the germination and growth of D. sanguinalis L. and A. retroflexus L.Using HPLC, one fraction with an absorption peak at 271 nm was separated from the crude toxin. This fraction could strongly inhibit the growth of D. sanguinalis L. at a concentration of 100 mg L-1 and could completely inhibit the seed germination of D. sanguinalis L. and A. retroflexus L. at a concentration of 50 mg L-1.关键词
Botrytis cinerea/toxin/ultraviolet mutation/herbicide activity/separation and purificationKey words
Botrytis cinerea/toxin/ultraviolet mutation/herbicide activity/separation and purification分类
农业科技引用本文复制引用
ZHANG Jin-lin,ZHANG Li-hui,LIU Ying-chao,MA Juan,LI Chuan,DONG Jin-gao..The Herbicidal Activity of Mutant Isolates from Botrytis cinerea[J].中国农业科学（英文版）,2006,5(8):622-628,7.基金项目
This study was supported by the Key R&D Program of Science and Technology Office of Hebei Province,China (03220142D). （03220142D）
