中国临床康复2004，Vol.8Issue(28)：6228-6229,2.
脑缺血再灌注后皮质区和纹状体区细胞周期蛋白D1和周期蛋白依赖性蛋白激酶4基国表达对神经细胞凋亡的影响
Effect of gene expressions of cyclin D1 and cyclin dependent kinase4 in cortex and striatum on apoptosis of nerve cells after cerebral ischemia reperfusion in rats
王少萍 1龚薇薇 2孙国岚 2王粤 2张红 2郭云良2
作者信息
- 1. 青岛大学医学院第二附属医院神经科,山东省青岛市,266042
- 2. 青岛大学医学院脑血管病研究所,山东省青岛市,266003
- 折叠
摘要
Abstract
BACKGROUND:The expressions of cyclins mainly play its apoptotic role after cerebral ischemia reperfusion and some apoptosis is caused by the abnormal regulation of cell proliferation cycle. OBJECTIVE:To investigate the relationship between the gene expressions of cyclin D1 and cyclin dependent kinase(CDK4) and neuronal apoptosis after focal cerebral ischemic reperfusion in rats. DESIGN:Randomized and controlled experimental research. SETTING and MATERIALS:This experiment was finished in the Institute of the Cerebrovascular Diseases,in Medical College of Qingdao University and the Key Laboratory of Cerebrovascular Diseases of Shandong Province.Thirty-six adult healthy female SD rats,with a body mass of 230 g to 270 g,of clearing grade,and purchased from Shanghai Experiment Animal Center of Chinese Science Academy.The animals were divided into sham-operated group(4 rats) and experiment groups(32 rats) which were further divided into ischemic 1.5 hours and reperfusion 2,6,12 hours,1,2,3,7 and 14 days subgroups,consisting of 4 rats each. METHODS: The experiment was performed by the authors.The model of focal ischemic reperfusion in rats was induced by occlusion of intraluminal middle cerebral artery with a nylon monofilament suture from left external internal carotid artery.The neuronal apoptosis was detected by terminal deoxynucleotidy1 transferase-mediated 5'-dUTP nick end- labeling(TUNEL)staining.Hybridization in situ was performed to examine the expressions of cyclin D1 mRNA and CDK4 mRNA. RESULTS: TUNEL positive neurons appeared from reperfusion 2 hours and peaked at 1 day and 2 days in cortex and striatum[(72.80± 4.66)and (96.75± 4.37) respectively].Cyclin D1 mRNA expression was detected in cortex and striatum of ischemic hemisphere as early as 2 hours after reperfusion while CDK4 mRNA was detected at 6 hours, and reached its highest levels at 12 hours and 1 day[in cortex(94.50± 2.75) and(85.75± 3.73)and in striatum(88.25± 5.06) and(89.80± 2.93) respectively],then decreased to sham-operated level at reperfusion 14 days.The expressions of cyclin D1 mRNA and CDK4 mRNA in the fields almost were the same as apoptosis. CONCLUSION:The cortex might be more sensitive to ischemia than striatum and the expressions of cyclin D1 and CDK4 mRNA might be one of the important factors inducing apoptosis in cerebral ischemia reperfusion.关键词
脑缺血/细胞/基因表达/细胞周期蛋白 D1/蛋白激酶类分类
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王少萍,龚薇薇,孙国岚,王粤,张红,郭云良..脑缺血再灌注后皮质区和纹状体区细胞周期蛋白D1和周期蛋白依赖性蛋白激酶4基国表达对神经细胞凋亡的影响[J].中国临床康复,2004,8(28):6228-6229,2.
