中国人兽共患病学报2009，Vol.25Issue(7)：623-626,4.
首次从国内鉴定到猪肠道杯状病毒及其分子流行病学调查
The first identification and molecular epidemiology of PEC in China
摘要
Abstract
A total of 189 stool samples from swine with diarrhea, collected in various porcine farms in the central region of China were tested for porcine enteric caliciviruses (PEC) member porcine sapoviruses (SaV) by reverse transcription polymerase chain reaction (RT-PCR) amplification using primers designed to detect porcine SaV. Selected amplicons were sequenced to establish phylogenetic relationships with reference strains. Porcine SaV were detected in 12.70% (24/189) of the samples. Phylogenetic studies based on partial RNA polymerase gene sequences indicated that the field strains of viruses isolated in China were closely related (75.6 88.3% identity) to the porcine SaV Cowden reference strain. These results provide evidence that caliciviruses of the genus sapovirus circulate in piglets in China, but further studies are needed to clarify their importance as cause of diarrhea. This is the first report of PEC in China.关键词
猪肠道杯状病毒/猪札如病毒/腹泻/猪Key words
porcine enteric caliciviruses/porcine sapovirus/diarrhea/swine分类
医药卫生引用本文复制引用
黄泽彬,汪镇南,黎满香,余兴龙,李润成,谢小雨,尹德明,颜运秋,白霞,刘忠华,丁建..首次从国内鉴定到猪肠道杯状病毒及其分子流行病学调查[J].中国人兽共患病学报,2009,25(7):623-626,4.基金项目
湖南省科技重大专项基金(2007FJ1003),湖南农业大学人才引进基金(04YJ03) （2007FJ1003）
