中国临床康复2004，Vol.8Issue(28)：6256-6257,2.
施万细胞移植对电针损伤大鼠中脑新生髓鞘的影响
Impact of Schwann cell transplantation on neogenetic myelin sheath of the midbrain in rats injured by electro-acupuncture
摘要
Abstract
BACKGROUND:The neogenesis of myelin sheath predicts the rehabilitative status of the injured nerves. OBJECTIVE:To observe whether there is neogenesis of myelin sheath after Schwann Cell(SC) transplantation in the midbrain injured by electroacupuncture. DESIGN:A completely randomised design. SETTINGS and MATERIALS:Study was conducted in Beijing Neurosurgical Institute. Wistar rats in either gender with a body mass of(180± 20) g were randomly allocated into electrical acupuncture injury group(control group) and SC transplantation group(SC group) with 18 rats each. INTERVENTION:SC obtained from the sciatic nerve of newborn rats was cultured in vitro and transplanted into the reticular structure in the midbrain of rats injured by electro-acupuncture after being marked by 5'Bromodexyuridine(BrdU).Specimens were stained by azure-methylene blue myelin sheath staining after immunohistochemical staining localization followed by semithin section.Neogenetic myelin sheath was observed under oil microscope.MAIN OUTCOME MEASURES:①The survival period of SC transplanted in the brain.② Neogenetic myelin sheath in both control group and SC group. RESULTS:Five rats in control group and 6 rats in SC group died during the study.Eighteen rats of each group were maintained into final analysis.BrdU-positive cell could be seen even at 8 months after SC transplantation, which mainly migrated towards cerebral cortex.Neogenetic myelin sheath could be seen in the injured brain stem at one month. CONCLUSION:SC transplantation can promote the regeneration of the injured central nervous system.关键词
细胞移植/中脑/髓鞘/再生分类
医药卫生引用本文复制引用
万虹,翟晶,历俊华,张亚卓,王忠诚..施万细胞移植对电针损伤大鼠中脑新生髓鞘的影响[J].中国临床康复,2004,8(28):6256-6257,2.基金项目
国家自然科学基金资助项目 (39970750) （39970750）
