黑龙江八一农垦大学学报2009，Vol.21Issue(3)：62-66,5.
哈茨木霉Rac基因的克隆及特征分析
Cloning and Characterization Analysis of Rac Gene From Trichoderma Harzianum
摘要
Abstract
In eukaryotes,Rac gene is crucial for rapid morphological responses to the environment.Rac gene was cloned by RT-PCR from Trichoderma harzianum cDNA. The gene encodes the protein of 204 amino acids with molecular mass of 22. 4 kD. With BlastP and bioinformational tools,it was revealed that amino acid sequences of Rac genes from some fungal species share higher similarity. The Rac from T. harzianum shares the highest similarity (82%) with that from Epichlos typhina and the lowest 75% with that from Talaromyces stipitatus. The GTP/GDP binding and effector domain of T. harzianum RAC protein were probably identified. The cDNA and deduced amino acid sequence of Rac gene were accepted by GeneBank (accession numbers: FJ595933 and ACM24223).关键词
哈茨木霉/Rac/基因克隆/特征分析Key words
trichoderma harzianum/rac/gene cloning/characterization analysis分类
农业科技引用本文复制引用
陈艳,丛华,王允,杨晓雪..哈茨木霉Rac基因的克隆及特征分析[J].黑龙江八一农垦大学学报,2009,21(3):62-66,5.基金项目
哈尔滨工业大学校基金(HIT.NSRIF.2008-18). （HIT.NSRIF.2008-18）
