摘要
Abstract
AIM: To investigate effects of Crocin on apoptosis of vascular endothelial cells injured by triol. METHODS: Apoptosis model induced by triol(cholestane-3β,5α,6β-triol)was adopted. Malondialdehyde (MDA) content was measured by colorimetric assay. The morphology and ultrastructure were detected by light microscope and electron microscope. DNA ladder was analyzed by DNA electrophoresis. The apoptosis rate was assessed with the flow cytometer assay. Bax mRNA expression of endothelial cells was measured by RT-PCR analysis. RESULTS: In Triol group, MDA content was dye deeply, mitochondria swelling and cavitations, mitochondrial cristae fragmentation, formation of apoptotic bodies, the DNA ladder in agarose gel and hypodiploid cusp was observed. Apoptosis rate was 30.62%. Bax mRNA expression increased. In the Crocin +Triol group, MDA content decreased, majority of cells grown in spreading out like that in control and group were 24.4%, 6.3%, respectively. Bax mRNA expression of endothelial Crocin cells in the crocin (10-6 mol·L-1) +Triol group decreased. CONCLUSION: maybe abnormally decrease apoptosis by anti-lipid peroxidation and adjust Bax mRNA expression.关键词
西红花苷/血管内皮细胞/Triol/凋亡/Bax/基因Key words
crocin/blood vessel endothelial cells/triol/apoptosis/Bax/gene分类
医药卫生
