国际眼科杂志2007，Vol.7Issue(3)：632-634,3.
水通道蛋白-1在牛角膜内皮细胞液体转运中作用研究
Research of the role of aquaporins-1 in corneal endothelial fluid transport
摘要
Abstract
AIM: To investigate the expression of aquaporins-1 (AQP-1)in cultured bovine corneal endothelial cells and to explore the role of AQP-1 in corneal endothelial fluid transport.METHODS:The bovine corneal cells were cultured in DMEM containing 200mL/L neonate bovine serum. AQP-1 expression in the bovine corneal endothelial cells was detected with immunohistochemistry method before and after treatment of the cells with aquaporin inhibitor, p-chloromercuribenzene sulfonate. The osmotic water permeability was determined bymonitoring volume changes of cultured bovine corneal endothelial cells.RESULTS: AQP-1 expression in the membrane of cultured bovine corneal endothelial cells was revealed by positive staining (in brown color). The reading of osmotic water permeability of the cultured bovine corneal endothelial cells before treatment with p-chloromercuribenzene sulfonate was 0.044±0.005cm/s, which significantly decreased to 0.017 0.003 cm/s(n= 15) after treatment.CONCLUSION: AQP-1 expressed in the membrane of cultured bovine corneal endothelial cells may play an important role in fluid transport of corneal endothelial cells. Alteration of the AQP-1 expression may cause abnormal corneal function and corneal edema.关键词
角膜内皮细胞/对氯汞苯磺酸酯/水通道蛋白-1Key words
corneal endothelium/p-chloromercuribenzene sulfonate/aquaporin-1分类
医药卫生引用本文复制引用
张莉,王莉..水通道蛋白-1在牛角膜内皮细胞液体转运中作用研究[J].国际眼科杂志,2007,7(3):632-634,3.基金项目
中国广东省深圳市科技项目(No.200603163)Shenzhen Science and Technology Foun dation of China (No. 200603163 ) （No.200603163）
