中国医学科学院学报2001，Vol.23Issue(1)：63-64,2.
Fudenine——一个新的与血糖调节相关的膜蛋白
Fudenine, Relative to Blood Glucose-control, Is a Novel Membrane Protein
常永生 1左瑾 1张雪峰 1张明 1方福德1
作者信息
- 1. 中国医学科学院中国协和医科大学基础医学研究所
- 折叠
摘要
Abstract
Objective To determine whether Fudenine is a novel membrane protein. Methods Green fluorescence protein(GFP)was used to localize Fudenine in vivo. GFP, as a control, was targeted to cytoplasm.Epithelial cell, CBRH7919, and non-epithelial cell, L-6TG, were cultured and transiently transfected by using the lipofectamine reagent. After 48 h, intact cells were examined with fluorescence microscope for Fudenine. Results Reporter plasmid pEGFP-N1, as a control , was expressed and localized to cytoplasm. But Fudenine, driven by the cytomegalovirus promoterenhancer contained in the pEGFP-N1 vector, was overexpressed and targeted to cellular membrane. Conclusions Fudenine is a novel membrane protein. It may play the similar role with its homologues AC133 antigen and prominin in human and mouse.respectively. It might be involved in signaling transduction and regulate blood glucose metabolism in vivo.关键词
Fudenine亚细胞定位细胞膜分类
生物科学引用本文复制引用
常永生,左瑾,张雪峰,张明,方福德..Fudenine——一个新的与血糖调节相关的膜蛋白[J].中国医学科学院学报,2001,23(1):63-64,2.
